primary antibodies against mmp 1  (Servicebio Inc)

Journal: Molecular Medicine Reports

Article Title: Tributyltin chloride induces chondrocyte damage through the activation of NLRP3‑mediated inflammation and pyroptosis

doi: 10.3892/mmr.2024.13247

Figure Lengend Snippet: TBTC induces chondrocyte damage in rats. (A) Cell Counting Kit-8 results showed that TBTC reduced rat chondrocyte viability in a concentration-dependent manner. (B) TBTC significantly increased LDH leakage from rat chondrocytes. (C) Reverse transcription-quantitative PCR results revealed that TBTC elevated NLRP3 mRNA expression levels. (D) Western blot analysis results revealed that TBTC upregulated the protein expression levels of NLRP3, IL-1β, IL-18, MMP-1 and MMP-13 in rat chondrocytes. *P<0.05, **P<0.01, ***P<0.001 vs. Con. Con, control; IL, interleukin; LDH, lactate dehydrogenase; MMP, matrix metalloproteinase; NLRP3, NLR family pyrin domain containing 3; TBTC, tributyltin chloride.

Article Snippet: Subsequently, primary antibodies against β-actin (1:1,000; cat. no. 4970; Cell Signaling Technology, Inc.), NLRP3 (1:1,000; cat. no. ab263899; Abcam), interleukin (IL)-1β (1:1,000; cat. no. ab315084; Abcam), IL-18 (1:1,000; cat. no. ab223293; Abcam), matrix metalloproteinase (MMP)-1 (1:500; cat. no. 70R-50066; AmyJet Scientific, Inc.), MMP-13 (1:1,000; cat. no. ab219620; Abcam), caspase-1 (1:1,000; cat. nos. ab286125 for rat or ab138483 for mouse; Abcam), PYD and CARD domain containing (ASC; 1:1,000; cat. no. ab307560; Abcam) and gasdermin D (GSDMD; 1:1,000; cat. no. ab219800; Abcam) were incubated with the PVDF membranes overnight at 4°C.

Techniques: Cell Counting, Concentration Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot

Journal: Molecular Medicine Reports

Article Title: Tributyltin chloride induces chondrocyte damage through the activation of NLRP3‑mediated inflammation and pyroptosis

doi: 10.3892/mmr.2024.13247

Figure Lengend Snippet: TBTC enhances the NLRP3-mediated inflammatory response and cellular pyroptosis in mouse OA. (A) TBTC increased the protein expression levels of NLRP3, IL-1β, IL-18, MMP-1 and MMP-13. (B) TBTC increased the expression levels of the pyroptosis-related proteins caspase-1, ASC and GSDMD in mouse osteoarticular tissues. *P<0.05, **P<0.01, ***P<0.001 vs. Con; #P<0.05, ##P<0.01, ###P<0.001 vs. OA. Con. ASC, PYD and CARD domain containing; Con, control; GSDMD, gasdermin D; IL, interleukin; MMP, matrix metalloproteinase; OA, osteoarthritis; TBTC, tributyltin chloride.

Article Snippet: Subsequently, primary antibodies against β-actin (1:1,000; cat. no. 4970; Cell Signaling Technology, Inc.), NLRP3 (1:1,000; cat. no. ab263899; Abcam), interleukin (IL)-1β (1:1,000; cat. no. ab315084; Abcam), IL-18 (1:1,000; cat. no. ab223293; Abcam), matrix metalloproteinase (MMP)-1 (1:500; cat. no. 70R-50066; AmyJet Scientific, Inc.), MMP-13 (1:1,000; cat. no. ab219620; Abcam), caspase-1 (1:1,000; cat. nos. ab286125 for rat or ab138483 for mouse; Abcam), PYD and CARD domain containing (ASC; 1:1,000; cat. no. ab307560; Abcam) and gasdermin D (GSDMD; 1:1,000; cat. no. ab219800; Abcam) were incubated with the PVDF membranes overnight at 4°C.

Techniques: Expressing

Journal: Journal of Microbiology and Biotechnology

Article Title: 1-Kestose Blocks UVB-Induced Skin Inflammation and Promotes Type I Procollagen Synthesis via Regulating MAPK/AP-1, NF-κB and TGF-β/Smad Pathway

doi: 10.4014/jmb.2311.11020

Figure Lengend Snippet: ( A, B ) Level of expressed MMP-1 was detected by western blotting 12 h after UVB irradiation. Band intensity was quantified by ImageJ and normalized to β-actin. ( C ) Dual-luciferase reporter assay was evaluated to measure the MMP-1 promoter activity in the HaCaT cells. The mRNA expression of ( D ) MMP-1, ( E ) MMP-3, ( F ) MMP-9, ( G ) COL1A1 and ( H ) COL2A1 was measured using RT-PCR, followed by treatment with 1-kestose (4, 6, and 10 mM) for 12 h. All data are expressed as mean ± SD ( n = 3). # p < .05, ## p < .01, ### p < .001, and #### p < .0001 vs. blank group, * p < .05, ** p < .01, *** p < .001, and **** p < .0001 vs control group.

Article Snippet: Primary antibodies against MMP-1 (#54376), ERK (#4695), JNK (#9252), p38 (#8690), p-ERK (#4370), p-JNK (#4671), p-p38 (#4511), c-Fos, (#2250), c-Jun (#9165), p-c-Fos (#5348), p-c-Jun (#3270), TGF-β1 (#3711), Smad2/3 (#8685), p-Smad2/3 (#8828), and β-actin (#4970), as well as horseradish peroxidase (HRP) goat anti-rabbit IgG (#2895) were purchased from Cell Signaling Technology (USA).

Techniques: Western Blot, Irradiation, Luciferase, Reporter Assay, Activity Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: PLOS ONE

Article Title: S -1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts

doi: 10.1371/journal.pone.0284713

Figure Lengend Snippet: HGF-1 cells were exposed to S1PC (300 μM) in the presence or absence of LPS (3 μg/mL) for 48 h. Bar graphs show the mRNA level of MMP-1 and -3, calculated relative to the level in each vehicle group. Values represent the mean ± S.D. (n = 5). ## p < 0.01.

Article Snippet: Other primary antibodies against MMP-1 (#54376), cleaved-ADAM17 (BS7064), and β-actin (PM053-7) were from Cell Signaling Technology (Danvers, MA, USA), Bioworld Technology (Bloomington, MN, USA) and MBL Life Science (Nagoya, Japan), respectively.

Techniques:

Journal: PLOS ONE

Article Title: S -1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts

doi: 10.1371/journal.pone.0284713

Figure Lengend Snippet: ( a ) HGF-1 cells were exposed to S1PC (300 μM) in the presence or absence of LPS (3 μg/mL) for the indicated times. Bar graphs show the mRNA level of MMP-1, calculated relative to the level in the vehicle group at each time point. Values represent the mean ± S.D. (n = 5). # p < 0.05, ## p < 0.01. ( b ) HGF-1 cells were exposed to S1PC in the presence or absence of LPS for 48 h at indicated concentrations. The graph shows the mRNA level of MMP-1 . Values represent the mean ± S.D. (n = 3). # p < 0.05, ## p < 0.01. ( c ) HGF-1 cells were exposed to S1PC (300 μM) in the presence or absence of LPS for the indicated times. Photographs show a representative result of Western blotting of MMP-1 with β-actin as an internal control. The graph shows the ratio of the MMP-1 band intensity relative to that of β-actin. Values represent the mean ± S.D. (n = 5). # p < 0.05, ## p < 0.01. ( d ) HGF-1 cells were exposed to S1PC in the presence or absence of LPS for 72 h. The graph shows the concentration of MMP-1 in media determined by ELISA normalized to the total cell protein amount in each treatment group. Values represent the mean ± S.D. (n = 3). ## p < 0.01.

Article Snippet: Other primary antibodies against MMP-1 (#54376), cleaved-ADAM17 (BS7064), and β-actin (PM053-7) were from Cell Signaling Technology (Danvers, MA, USA), Bioworld Technology (Bloomington, MN, USA) and MBL Life Science (Nagoya, Japan), respectively.

Techniques: Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: PLOS ONE

Article Title: S -1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts

doi: 10.1371/journal.pone.0284713

Figure Lengend Snippet: ( a ) HGF-1 cells were exposed to S1PC (300 μM) in the presence or absence of LPS (3 μg/mL) for the indicated times. Photographs show a representative result of Western blotting of phosphorylated EGFR (pEGFR) and EGFR with β-actin as an internal control. Bar graphs show the ratio of the pEGFR band intensity relative to that of EGFR and β-actin, calculated relative to the level at 0 min. Values represent the mean ± S.D. (n = 4). ## p < 0.01. ( b ) HGF-1 cells were exposed to EGFR tyrosine kinase inhibitor AG-1478 (0.1 nM) in the presence or absence of LPS for 48 h. Photographs show a representative result of Western blotting of MMP-1 with β-actin as an internal control. Bar graphs show the ratio of the MMP-1 band intensity relative to that of β-actin. Values represent the mean ± S.D. (n = 3). ## p < 0.01.

Article Snippet: Other primary antibodies against MMP-1 (#54376), cleaved-ADAM17 (BS7064), and β-actin (PM053-7) were from Cell Signaling Technology (Danvers, MA, USA), Bioworld Technology (Bloomington, MN, USA) and MBL Life Science (Nagoya, Japan), respectively.

Techniques: Western Blot

Journal: PLOS ONE

Article Title: S -1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts

doi: 10.1371/journal.pone.0284713

Figure Lengend Snippet: ( a ) HGF-1 cells were exposed to S1PC (300 μM) in the presence or absence of LPS (3 μg/mL) for the indicated times. Photographs show a representative result of Western blotting of the active form of TACE with β-actin as an internal control. Bar graphs show the ratio of the active form of TACE band intensity relative to that of β-actin, calculated relative to the level at 0 min. Values represent the mean ± S.D. (n = 4). ## p < 0.01. ( b ) HGF-1 cells were exposed to an TACE inhibitor TAPI-1 (1 μM) in the presence or absence of LPS for 48 h. Photographs show a representative result of Western blotting of MMP-1. Bar graphs show the ratio of the MMP-1 band intensity relative to that of β-actin. Values represent the mean ± S.D. (n = 3). # p < 0.05.

Article Snippet: Other primary antibodies against MMP-1 (#54376), cleaved-ADAM17 (BS7064), and β-actin (PM053-7) were from Cell Signaling Technology (Danvers, MA, USA), Bioworld Technology (Bloomington, MN, USA) and MBL Life Science (Nagoya, Japan), respectively.

Techniques: Western Blot

Journal: PLOS ONE

Article Title: S -1-propenyl-L-cysteine suppresses lipopolysaccharide-induced expression of matrix metalloproteinase-1 through inhibition of tumor necrosis factor-α converting enzyme-epidermal growth factor receptor axis in human gingival fibroblasts

doi: 10.1371/journal.pone.0284713

Figure Lengend Snippet: The binding of LPS to TLR4 activates TACE and this activation promotes the phosphorylation of EGFR to induce the expression of MMP-1 in HGF-1 cells. Both EGFR tyrosine kinase and TACE inhibitors, AG-1478 and TAPI-1, antagonize the LPS-induced expression of MMP-1. S1PC suppresses the LPS-induced activation of TACE and subsequent phosphorylation of EGFR, thus ultimately suppressing the expression of MMP-1. TACE: tumor necrosis factor-α converting enzyme, EGFR: epidermal growth factor receptor, LPS: lipopolysaccharide, MMP-1: matrix metalloproteinase-1, S1PC: S -1-propenyl-L-cysteine, TLR4: toll-like receptor 4.

Article Snippet: Other primary antibodies against MMP-1 (#54376), cleaved-ADAM17 (BS7064), and β-actin (PM053-7) were from Cell Signaling Technology (Danvers, MA, USA), Bioworld Technology (Bloomington, MN, USA) and MBL Life Science (Nagoya, Japan), respectively.

Techniques: Binding Assay, Activation Assay, Expressing